Abstract
BackgroundHepatocellular carcinoma (HCC) is one of the leading causes of cancer death in China. This study investigated the effects of Annexin A7 (ANXA7) on the inhibition of HCC lymph node metastasis in a mouse model.MethodsThe stable knockup and knockdown of Annexin A7-expressing HCC cells using Annexin A7 cDNA and shRNA vectors, respectively, were injected into a mouse footpad to establish primary and metastatic tumors in mice. On the 14th, 21st, and 28th days after HCC cells inoculation, the mice were sacrificed for inspection of primary and secondary tumors and immunohistochemistry of Annexin A7 expression.ResultsThe lymph node metastasis rate of the FANXA7-control group was 77%, and the lymph node metastasis rate of the FANXA7-down group was 100% (p < 0.05). In contrast, the lymph node metastasis rate of the PANXA7-up group was 0% and that of the PANXA7-control group was 36% (p < 0.05). Furthermore, immunohistochemistry experiments revealed that the subcellular localization of Annexin A7 protein in both primary and lymph node-metastasized tumors was mainly in the cytosol. In addition, the expression of the 47 kDa and 51 kDa isoforms of Annexin A7 protein changed during tumor progression.ConclusionThis study indicated that Annexin A7 expression was able to inhibit HCC lymph node metastasis, whereas knockdown of Annexin A7 expression significantly induced HCC metastasis to local lymph nodes.
Highlights
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer death in China
Western blot results indicated that the expression of Annexin A7 in FANXA7-down was significantly lower than FANXA7-control, but Annexin A7 expression in Hca-F showed no difference compared to FANXA7-control (Figure 1C)
These data showed that HcaF cells had downregulated expression of Annexin A7, at the gene level and at the protein level
Summary
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer death in China. This study investigated the effects of Annexin A7 (ANXA7) on the inhibition of HCC lymph node metastasis in a mouse model. Hepatocellular carcinoma (HCC), the most common type of liver cancer, is a significant health problem in the world due to its high incidence and mortality rate. HCC accounts for more than 700,000 new cases and over 500,000 deaths each year worldwide. HCC is heterogeneous and a highly aggressive malignancy; to date, there are no effective means for a cure, due to high invasion, early metastasis, and high tumor recurrence after surgery or interventional treatment. Early detection and prevention of HCC and the control of HCC metastasis are urgently needed to improve HCC prognosis.
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