Ankara ili kışlık sebze üretim alanlarında Cauliflower mosaic virus’u ve vektörlerinin serolojik ve moleküler olarak tanılanması

Abstract

Ankara province is one of the major planting area of winter vegetables (Brassicaceae), such as cauliflower, broccoli, white cabbage, red cabbage and radish. Periodic surveys were carried out in Centrum, Ayaş, Beypazarı, Nallıhan and Çubuk districts of Ankara province, which are the main winter vegetable production areas in Ankara; thus samples were collected from cabbage, broccoli, radish, and cauliflower plants between February and October 2016 and 2017. In this study, as viral diseases in winter vegetables, Cauliflower mosaic virus (CaMV) and those vectors were detected serologically by DAS-ELISA and molecular by PCR. As a result, among 271 collected plant samples tested by the DAS-ELISA method only 0.03% were positively infected with CaMV, while 15.13% of samples were found to be infected with CaMV; by PCR. Three population of Brevicoryne brassicae aphids were also collected from infected plant samples determined as infected with CaMV. Statistical analysis was made after the determination of infected plants number, then P-value was determined as 0.0001. According to the results of present research, CaMV was present in the Centrum, Ayaş, Beypazarı, Nallıhan districts and not present in Çubuk district of Ankara. This is the first report on the presence of the CaMV, a DNA virus and its vector, B. brassicae in Ankara province.