Anisotropic diffusion of oxygen in slices of rat muscle.

Abstract

The diffusion coefficient of oxygen (D) and the fluorescence quenching coefficient (K') of pyrenebutyric acid (PBA) were measured in sections of rat hamstring muscles. Values of D and K' at temperatures (Tc) of 20, 30, and 40 degrees C were determined and referred to the values in water. In sections cut parallel to the muscle fibers, D = DH2O (0.380 +/- 0.038), whereas in sections cut across the grain of the fibers, D = DH2O (0.985 +/- 0.039). Oxygen diffuses along the length of a muscle fiber over twice as rapidly as it diffuses in directions perpendicular to the long axis of the fiber. This suggests that fibers, myofibrils, or myofilaments offer substantial barriers to the diffusion of oxygen, whereas extracellular space and spaces around fibers or myofibrils or myofilaments offer no more resistance than water to the diffusion of oxygen. Corresponding estimates for K' were K' = K'H2O[0.14 (1 + 0.25 Tc)] and K' = K'H2O[0.21 (1 + 0.25 Tc)] for slices cut parallel to the long axis of muscle fibers and across the long axis, respectively. Standard deviations of K' were 9%.