Abstract

Application of Luft's ruthenium red technique to extraocular muscle of Fundulus grandis reveals dense accumulations of ruthenium red-positive material along the sarcolemma, in the synaptic cleft of the myoneural junction and in the T tubules. The granular material in the terminal sacs of the sarcoplasmic reticulum (SR), which is consistently preserved by glutaraldehyde fixation and osmium tetroxide postfixation, also reacts with ruthenium red. The intracellular ruthenium red has been seen outside the SR only where the sarcolemma obviously has been disrupted.

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