Abstract
Anion transport in sickle cells (SS RBC) mediated by the band 3 membrane protein was evaluated by three different measures in both oxygenated and deoxygenated conditions and compared to normal red cells. First, Cl- self-exchange measured as 36Cl- efflux at 0 degrees C was normal in SS RBC in both Vmax and dependence on extracellular Cl- concentration. There was no effect of deoxygenation on either parameter. Second, stilbene-sensitive 35SO4=; SO4= exchange, measured at 37 degrees C where morphologic sickling occurred, was also unaffected by deoxygenation and was normal compared to normal red cells. Third, conductive Cl- flux was assessed by measuring the rates of Cl(-)-limited K+ efflux in valinomycin-treated cells at 37 degrees C. Both the stilbene-sensitive and insensitive components of net Cl- flux were similar in SS RBC and normal red cells, and were unaltered by morphologic sickling. Thus, despite dramatic alterations in cation transport in SS RBC and the demonstration of interaction between band 3 protein and sickle cell, anion transport functions appear to be normal in SS RBC and are unaffected by deoxygenation. These data suggest that the majority of the anion exchangers in SS RBC are functionally normal.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.