Abstract
New strategies to economically manufacture large quantities of vaccines in less time are greatly needed to cater for the increasing global human population. This study aimed at developing a strategy to efficiently manufacture a highly purified plasmid vaccine via anion exchange monolithic chromatography. Diethylamine and triethylamine were used to provide the functional groups for polymethacrylate monoliths and used in anion exchange chromatography of plasmid pcDNA3F. Five chromatographic settings as described in the first table of this article were studied and it was concluded that the triethylamine functionalised conical monolith combined with optimum buffers’ and pH conditions produced the highest quality of pcDNA3F. Plasmid yield (3003.55 mg/L), plasmid recovery (90.25%), protein (0.01 mg/L), LPS (0.12 EU/mg) with no detectable gDNA and RNA were obtained at a low NaCl concentration of 0.25 M. Apparently, this technology will have a great impact on the overall plasmid vaccine production and particularly on the development of axial flow monolithic plasmid vaccine purification.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
