Anion and cation channels in the basolateral membrane of rabbit parietal cells

Abstract

Ion channels in the basolateral membrane of rabbit parietal cells in isolated gastric glands were studied by the patch clamp technique. Whole-cell current-clamp recordings showed that the membrane potential (Em) changed systematically as a function of the chloride concentrations of the basolateral bathing solution ([Cl-]0), and of the pipette (intracellular) solution. The relationship between Em and [Cl-]0 was not affected by additions of histamine, dibutyryl-cAMP, 4-acetoamido-4'-isothiocyanostilbene-2,2'-disulfonic acid and diphenylamine-2-carboxylate. The whole-cell Cl- conductance was insensitive to voltage. In cell-attached and cell-free patch membranes, however, single Cl- channel opening events could not be observed. The value of Em depended little on the basolateral K+ concentration, but inward-rectifier K+ currents were observed in the whole-cell configuration, activated by hyperpolarizing pulses and inhibited by extracellular Ba2+. In cell-attached and cell-free patches, openings of single inward-rectifier K+ channels and non-selective cation channels were infrequently recorded. Neither cAMP nor Ca2+ activated these cation channels. The single K+ channel conductance was about 230 pS under the symmetrical high K+ conditions and was inhibited by intracellular tetraethylammonium ions (TEA). The non-selective cation channel had a voltage-independent single conductance of 22 pS and was not inhibited by TEA.