Animal-Based Model to Investigate the Minimum Tumor Size Detectable with an Electrical Impedance Scanning Technique

Abstract

Rationale and Objectives The purpose of this study was to determine the minimum tumor size detectable with electrical impedance scanning (EIS) in laboratory animals. Materials and Methods VX2 tumor cells (1 mm 3) were implanted bilaterally into the upper leg musculature of five white New Zealand rabbits. EIS and ultrasound (US) were performed before, during, and immediately after implantation and on every 2nd day thereafter until tumors could be visualized with both modalities. This was followed by an extended follow-up regimen until a tumor size of 1 cm 3 was reached. Rabbits were anesthetized subcutaneously. Results Tumors could not be implanted in one rabbit. Neither EIS nor US were performed in one rabbit due to severe skin alterations at the implantation site. No focal increase in conductance was visible before or immediately after tumor injection. The smallest tumor identified with EIS was determined with US to be approximately 8 mm 3. The mean tumor size at initial detection was 52 mm 3 (determined with EIS). In two cases, the tumor was first seen with US (EIS detection was delayed with a delay of 1 and 2 days, respectively). In all remaining cases, tumors were detected simultaneously with both EIS and US. All tumors were visible with EIS. Conclusion The animal-based model is feasible. VX2 tumors are detectable with EIS. Tumors characterized by a focal spot at EIS could be detected starting at a tumor size of 7.5 mm 3.