Animal study on primary dysmenorrhoea treatment at different administration times.

Bao-Chan Pu,Rui Liu,Li-Na Gao,Ai-Zhu Li,Ling Fang

doi:10.1155/2015/367379

Abstract

The new methods of different administration times for the treatment of primary dysmenorrhea are more widely used clinically; however, no obvious mechanism has been reported. Therefore, an animal model which is closer to clinical evaluation is indispensable. A novel animal experiment with different administration times, based on the mice oestrous cycle, for primary dysmenorrhoea treatment was explored in this study. Mice were randomly divided into two parts (one-cycle and three-cycle part) and each part includes five groups (12 mice per group), namely, Jingqian Zhitong Fang (JQF) 6-day group, JQF last 3-day group, Yuanhu Zhitong tablet group, model control group, and normal control group. According to the one-way ANOVAs, results (writhing reaction, and PGF2α, PGE2, NO, and calcium ions analysis by ELISA) of the JQF cycle group were in accordance with those of JQF last 3-day group. Similarly, results of three-cycle continuous administration were consistent with those of one-cycle treatment. In conclusion, the consistency of the experimental results illustrated that the novel animal model based on mice oestrous cycle with different administration times is more reasonable and feasible and can be used to explore in-depth mechanism of drugs for the treatment of primary dysmenorrhoea in future.

Highlights

Dysmenorrhoea, which is caused by female endocrine disorders, is one of the unresolved problems in medical science
We aimed to explore and establish an animal model by evaluating primary dysmenorrhoea treatment at different administration times, in which the primary dysmenorrhoea model was based on the proestrus stage of mice
Given the preliminary results of this experimental study, further clinical and animal experimental studies will be conducted. Both in one-cycle and three-cycle experiments, comparing with the model control group, the writhing times and contents of PGF2α and calcium ion in serum were significantly decreased by administration of Jingqian Zhitong Fang (JQF) or Yuanhu Zhitong tablet

Summary

Introduction

Dysmenorrhoea, which is caused by female endocrine disorders, is one of the unresolved problems in medical science. 80% of women globally had dysmenorrhoea in different degrees according to a report from the British medical authority [1]. This condition is classified into two categories: primary and secondary dysmenorrhoea. The incidence of primary dysmenorrhoea is higher. Primary dysmenorrhoea can be mostly experienced by young women, which occurs without an identifiable pelvic pathology. Such condition is mainly characterised by cramping pain in the lower abdomen immediately before or during menstruation, which does affect the quality of their life and work [3]

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Discussion

Conclusion