Animal in Vivo Studies and In Vitro Experiments with Human Tubes for End-To-End Anastomotic Operation by a CO2-Laser Technique

Abstract

A CO2-laser technique for reanastomosis was carried out in 4 groups of 10 rabbits testing different laser performances between 5 and 14 watts and different exposure times from .1-.5 seconds. Optimal energy density was found in Group 3 which was exposed to 64 watts/sq. cm. In this group 70% achieved successful reanastomosis i.e. an uncomplicated pregnancy with live-born young at term. With the laser technique only the serosa and subserosa are coagulated allowing the deeper tissue layers (muscularis and mucosa) to adapt without tension. Because there is no foreign body stimulus as there is with sutures tissue reaction is minimized. Not only is the laser microsurgery successful in reversing sterilization without additional techniques the complete operation in the animal experiments including the initial laparotomy required no more than 20 minutes lessening the danger of secondary infections and anesthetic risk.