Animal experimental study on the biocompatibility of the composite materials for artificial airtube

Abstract

Objective To evaluate the biocompatibility of the composite materials for making artificial airtube. Methods Skin hypersusceptibility test: 40 male guinea pigs were divided into 5 groups (n= 8), including nano-carbon fibers group, collagen protein/nano- hydroxyapatite (HAC) group, mixed materials group, positive control (formaldehyde) group and negative control (normal saline) group. Skin allergic response induced by the leaching liquor of each material was observed. Pyrogen test: 12 New Zealand rabbits were divided into 4 groups (n=3), including nano-carbon fibers group, collagen protein/nano-HAC group, mixed materials group and negative control (normal saline) group. Pyrogen in the leaching liquor of each material was examined. Hemolysis test was used to investigate the effect of the leaching liquor of each material on rabbit' s blood cells hemolysis rate. Results Sensibilization rate of each material was 0. There was no significant difference in statistics compared with negative control group(P>0.05 ). In pyrogen test, the fervescence of the animals in each group were (0.3±0.2), (0.2±0.1) and (0.3±0.2) ℃, respectively (all less than 0.6℃ ), and the sum total of fervescence was less than 1.4℃. The hemolysis rates of all groups were (0.130 1±0.000 4)%, (0.200 4±0.000 4)% and(0.150 2±0.000 4)% (all<5%). Conclusions Consistent materials for new artificial airtube are conformed to safety standard by biocompatibility test. The results provide evidences to further study of animal experimental model establishment. Key words: Trachea; Artificial organs; Bioeompatible materials; Animal experimentation; Hemolysis