Abstract
1. 1. The chirality of astaxanthin from the carotenoproteins of Homarus gammarus, crustacyanin (lobster shells) and ovoverdin (lobster eggs), has been examined by the camphanate method. Crustacyanin contained (3S,3′S)- axtaxanthin , (3R,3′S, meso)- astaxanthin and (3R,3′R)- astaxanthin in relative proportion 33:28:39. Astaxanthin ex ovoverdin consisted of the three optical isomers in ratio 30:28:42, closely similar to the ratio 31:25:44 found for total lobster egg astaxanthin. 2. 2. Individual recombination studies with apocrustacyanin and the natural astaxanthin mixture, the three pure, synthetic, optical isomers of astaxanthin and (3S)- adonirubin are reported. The recombined carotenoproteins were characterized by electronic and CD spectra and approximate mol. wt determined by ultrafiltration. 3. 3. The recombination method used resulted in carotenoproteins of α-crustacyanin size. The semisynthetic carotenoproteins obtained by recombination with each of the three astaxanthin isomers or (3S)- adonirubin showed only minor differences in their electronic and CD spectra relative to those of native α-crustacyanin. It is concluded that the chirality of the prosthetic group is not critical. 4. 4. No recombination between apo-ovoverdin and astaxanthin could be achieved. The CD properties for ovoverdin are discussed.
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More From: Comparative Biochemistry and Physiology -- Part B: Biochemistry and Molecular Biology
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