Animal Biomedicine Controls Root Knot Disease in Lentil Callus Culture: Enriched Advanced Clinical Toxicology Socio Economy Science Technology Communication by Preventing 21st Century COVID-19 Like Pandemic Diseases

Abstract

The economic pulse seeds, Lens esculentum L. Cv. ‘Asha’ root callus develop on Murashige and Skoog (MS)-media supplemented with 2 mg/1, 2,4-D (2,4-dichloro phenoxy acetic acid) and inoculated with Meloidogyne incognita larvae (65 ± 2 J2) in the culture tube to study their multiplication rate under sterilized condition in the tissue culture laboratory, and biomedicine-nematode extract (NE) prepared from M. incognita females, when applied at 1.3mg/culture tube to root callus of lentil, reduced nematode infestation of callus and improved callus growth. This animal biomedicine-NE is thought to induce defense-resistance in lentil root callus and may prevent 21st –century pandemics like virus diseases by boosting immunity, resisting toxic effects, and improved biomedicine, advanced in clinical toxicology, green-socio-economy, science technology, and communication application. The biomedicine-NE not only increase agriculture and crop production but also enable the development of a new-generation of nanotechnology-agrochemicals reducing negative-environmental-impacts and maintaining-crop-yields. And both; biomedicine-NE and pulses may itself focus on nanoformulation of agrochemicals and other nanotechnological interdisciplinary future nanotoxicology research and applications in agriculture, and interaction nanomaterials-NE with plants, their environment, and other organisms also.