ANGUSTIFOLIA is a central component of tissue morphogenesis mediated by the atypical receptor-like kinase STRUBBELIG

Yang Bai,Hemal Bhasin,Prasad Vaddepalli,Lynette Fulton,Kay Schneitz,Martin Hülskamp

doi:10.1186/1471-2229-13-16

Abstract

BackgroundDuring plant tissue morphogenesis cells have to coordinate their behavior to allow the generation of the size, shape and cellular patterns that distinguish an organ. Despite impressive progress the underlying signaling pathways remain largely unexplored. In Arabidopsis thaliana, the atypical leucine-rich repeat receptor-like kinase STRUBBELIG (SUB) is involved in signal transduction in several developmental processes including the formation of carpels, petals, ovules and root hair patterning. The three STRUBBELIG-LIKE MUTANT (SLM) genes DETORQUEO (DOQ), QUIRKY (QKY) and ZERZAUST (ZET) are considered central elements of SUB-mediated signal transduction pathways as corresponding mutants share most phenotypic aspects with sub mutants.ResultsHere we show that DOQ corresponds to the previously identified ANGUSTIFOLIA gene. The genetic analysis revealed that the doq-1 mutant exhibits all additional mutant phenotypes and conversely that other an alleles show the slm phenotypes. We further provide evidence that SUB and AN physically interact and that AN is not required for subcellular localization of SUB.ConclusionsOur data suggest that AN is involved in SUB signal transduction pathways. In addition, they reveal previously unreported functions of AN in several biological processes, such as ovule development, cell morphogenesis in floral meristems, and root hair patterning. Finally, SUB and AN may directly interact at the plasma membrane to mediate SUB-dependent signaling.

Highlights

During plant tissue morphogenesis cells have to coordinate their behavior to allow the generation of the size, shape and cellular patterns that distinguish an organ
* Correspondence: schneitz@wzw.tum.de †Equal contributors 2Entwicklungsbiologie der Pflanzen, Wissenschaftszentrum Weihenstephan, Technische Universität München, Emil-Ramann-Str. 4, Freising 85354, Germany Full list of author information is available at the end of the article In Arabidopsis, intercellular signaling mediated by the atypical leucine-rich repeat transmembrane receptor-like (LRR-RLK) STRUBBELIG (SUB) is essential for a number of developmental processes [1,2,3,4,5,6]
Taken together the results indicated that STRUBBELIG-LIKE MUTANT (SLM) genes contribute to SUB signal transduction

Summary

Introduction

During plant tissue morphogenesis cells have to coordinate their behavior to allow the generation of the size, shape and cellular patterns that distinguish an organ. In Arabidopsis thaliana, the atypical leucine-rich repeat receptor-like kinase STRUBBELIG (SUB) is involved in signal transduction in several developmental processes including the formation of carpels, petals, ovules and root hair patterning. In plants the coordination of cellular behavior within a tissue is intrinsically linked to cell wall biogenesis and dynamics, as plant cells are connected through semi-rigid cell walls that drastically limit their relative movement It is a major current challenge in plant biology to understand the mechanistic basis of intercellular communication and its connection to the cell wall during tissue morphogenesis. Sub mutants exhibit twisted stems, petals and carpels/siliques These phenotypes indicate a role for SUB in the control of integument initiation and outgrowth as well as stem and floral organ shape [1,2,6]. SUB plays a role in internode length (and stem height), a trait that is potentially important for optimizing yield in crop plants

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