Angustibacter aerolatus sp. nov., isolated from air

Abstract

A novel actinomycete strain, 7402J-48(T), was isolated from an air sample collected from Jeju island, Republic of Korea. Cells were gram-positive, aerobic, flagellated, short rods. Strain 7402J-48(T) showed highest 16S rRNA gene sequence similarity (98.6 %) with Angustibacter luteus TT07R-79(T), and had relatively low sequence similarities (below 95.1 %) with other members of the family Kineosporiaceae. The cell wall of strain 7402J-48(T) contained alanine, glutamic acid and 2,6-diaminopimelic acid, suggesting A1γ-type peptidoglycan. The menaquinones were MK-9(H(4)) and MK-8(H(4)). The acyl type of the cell-wall muramic acid was acetyl. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and an unknown lipid were present. The cellular fatty acid profile comprised a large amount of anteiso-C(15 : 0), moderate amounts of C(16 : 0), summed feature 3 (including C(16 : 1)ω7c and/or C(16 : 1)ω6c), C(17 : 0), iso-C(15 : 0), C(16 : 0) N alcohol and C(17 : 1)ω8c, and small amounts of other fatty acids. The DNA G+C content of strain 7402J-48(T) was 73 mol%. Based on its phenotypic and genotypic characteristics, strain 7402J-48(T) ( = KACC 15527(T) = NBRC 108730(T)) is proposed as the type strain of a novel species, Angustibacter aerolatus sp. nov. An emended description of the genus Angustibacter is provided.