Abstract
.Lymph node biopsy is a primary means of staging breast cancer, yet standard pathological techniques are time-consuming and typically sample less than 1% of the total node volume. A low-cost fluorescence optical projection tomography (OPT) protocol is demonstrated for rapid imaging of whole lymph nodes in three dimensions. The relatively low scattering properties of lymph node tissue can be leveraged to significantly improve spatial resolution of lymph node OPT by employing angular restriction of photon detection. It is demonstrated through porcine lymph node metastases models that simple filtered-backprojection reconstruction is sufficient to detect and localize -diameter metastases (the smallest clinically significant) in 1-cm-diameter lymph nodes.
Highlights
JBO LettersTorres,a Chengyue Li,a Yusheng He,a Lagnojita Sinha,a Georgia Papavasiliou,a Husain A
Lymph node biopsy is a primary means of staging breast cancer, yet standard pathological techniques are time-consuming and typically sample less than 1% of the total node volume
While small numerical aperture (NA) is common in standard optical projection tomography (OPT) to maximize depth of field, the level required for scatter rejection as demonstrated in the aforementioned study is an order of magnitude lower than what is typically used
Summary
Torres,a Chengyue Li,a Yusheng He,a Lagnojita Sinha,a Georgia Papavasiliou,a Husain A. AIllinois Institute of Technology, Department of Biomedical Engineering, Chicago, Illinois, United States bUniversity of Chicago, Department of Pathology, Chicago, Illinois, United States cIllinois Institute of Technology, Department of Electrical and Computer Engineering, Chicago, Illinois, United States
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