Angiotensin II modulates the cardiovascular responses to microinjection of NPY Y 1 and NPY Y 2 receptor agonists into the nucleus tractus solitarii of the rat

Abstract

The aim of this work was to investigate the modulation of the cardiovascular effects of neuropeptide Y (NPY) in the nucleus tractus solitarii (NTS) by angiotensin II (Ang II) and to determine the NPY receptor subtype involved in this modulation. Anesthesized Sprague–Dawley rats received microinjections in the NTS of Ang II (threshold and ED 50 doses) with NPY Y 1 agonist Leu 31Pro 34NPY and NPY Y 2 agonist NPY(13–36) (threshold and ED 50 doses). The changes in mean arterial pressure (MAP) and heart rate (HR) recorded in the femoral artery were analyzed during 60 min after the microinjections. The injection of threshold doses of Ang II, Y 1 agonist or Y 2 agonist alone did not produce any change in cardiovascular parameters. However, the co-injections into the NTS of threshold doses of both Ang II and the Y 1 agonist elicited significant increases of MAP and HR of about 12 and 10%, respectively. The co-administration of threshold doses of Ang II with the Y 2 agonist also induced a significant vasopressor response. The vasodepressor and bradycardiac effect of an ED 50 dose of the Y 1 agonist was significantly counteracted ( P<0.01) by a threshold dose of Ang II. The vasopressor effect elicited by an ED 50 dose of the Y 2 agonist was significantly enhanced by a threshold dose of Ang II ( P<0.01). No significant change of cardiovascular responses elicited by an ED 50 dose of Ang II was observed in the presence of threshold doses of the Y 1 agonist or of the Y 2 agonist. The present study gives functional evidences for a differential modulatory activity of Ang II on the cardiovascular responses mediated by Y 1 and Y 2 receptor subtypes, which may be of relevance for central cardiovascular regulation in the NTS.