Abstract
The effects of angiotensin II on cytosolic free Ca 2+ ion concentrations ([Ca 2+] i were studied in single porcine granulosa cells using the calcium-sensitive fluorescent dye fura-2 and high temporal resolution fluorescent videomicroscopy. Angiotensin II initiated specific, rapid, transient and topographically organized increases in [Ca 2+] i in a subpopulation of single swine granulosa cells. The Ca 2+ source for this angiotensin II-mediated [Ca 2+] i transient appeared to be internal stores, and a pertussis toxin-sensitive guanine nucleotide binding protein was implicated in this receptor-mediated Ca 2+ rise. Our single-cell studies also revealed a striking functional heterogeneity among granulosa cells, since folliclestimulating hormone-responsive cells were not angiotensin II responsive. We conclude that single swine granulosa cells are targets of specific angiotensin II action on intracellular pools of Ca 2+.
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