Angiotensin II-induced Hypertension is Reduced by Deficiency of P-selectin Glycoprotein Ligand-1

Qian Wang,Yingxian Sun,Daniel T Eitzman,Chiao Guo,Kyle Kleiman,Jessica Venugopal,Hui Wang,Jintao Wang

doi:10.1038/s41598-018-21588-3

Abstract

Identification of inflammatory mediators that regulate the vascular response to vasopressor molecules may aid in the development of novel therapeutic agents to treat or prevent hypertensive vascular diseases. Leukocytes have recently been shown to be capable of modifying blood pressure responses to vasopressor molecules. The purpose of this study was to test the hypothesis that deficiency of the leukocyte ligand, Psgl-1, would reduce the pressor response to angiotensin II (Ang II). Mice deficient in Psgl-1 (Psgl-1−/−) along with wild-type (WT) controls were treated for 2 weeks with a continuous infusion of Ang II. No differences in blood pressure between the groups were noted at baseline, however after 5 days of Ang II infusion, systolic blood pressures were higher in WT compared to Psgl-1−/− mice. The pressor response to acute administration of high dose Ang II was also attenuated in Psgl-1−/− compared to WT mice. Chimeric mice with hematopoietic deficiency of Psgl-1 similarly showed a reduced pressor response to Ang II. This effect was associated with reduced plasma interleukin-17 (IL-17) levels in Psgl-1−/− mice and the reduced pressor response was restored by administration of recombinant IL-17. In conclusion, hematopoietic deficiency of Psgl-1 attenuates Ang II-induced hypertension, an effect that may be mediated by reduced IL-17.

Highlights

P-selectin glycoprotein ligand-1 (Psgl-1) is a leukocyte ligand that binds to selectins and mediates tissue recruitment of leukocytes and platelets[8]
Blood pressure response to angiotensin II (Ang II) is attenuated in Psgl-1 deficient mice
Systemic infusion of Ang II increased blood pressure as measured by tail-cuff plethysmography and carotid artery catheterization in both WT and Psgl-1−/− mice compared with saline-infused control mice

Summary

Introduction

P-selectin glycoprotein ligand-1 (Psgl-1) is a leukocyte ligand that binds to selectins and mediates tissue recruitment of leukocytes and platelets[8]. Psgl-1 is required for sequential recruitment and generation of Th17 T cells in some models of inflammation[9] suggesting Psgl-1 could contribute to regulation of vascular tone. Deficiency of Psgl-1 has previously been shown to lead to endothelial cytokine resistance due to attenuation of endothelial NF-κB activation[10]. Since Ang II has been shown to signal through NF-κB11, we tested the hypothesis that deficiency of Psgl-1 would attenuate the pressor response to Ang II

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Conclusion