Angiotensin-I-Converting Enzyme Inhibitory and Antioxidant Activities of Protein Hydrolysate from Muscle of Barbel (Barbus callensis)

Assaad Sila,Anissa Haddar,Oscar Martinez-Alvarez,Ali Bougatef

doi:10.1155/2013/545303

Abstract

The present study investigated angiotensin-I-converting enzyme (ACE) inhibitory and antioxidant activities of barbel muscle protein hydrolysate prepared with Alcalase. The barbel muscle protein hydrolysate displayed a high ACE inhibitory activity (CI50=0.92 mg/mL). The antioxidant activities of protein hydrolysate at different concentrations were evaluated using variousin vitroantioxidant assays, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical method and reducing power assay. The barbel muscle protein hydrolysate exhibited an important radical scavenging effect and reducing power. These results obtained byin vitrosystems obviously established the antioxidant potency of barbel hydrolysate to donate electron or hydrogen atom to reduce the free radical. Furthermore, these bioactive substances can be exploited into functional foods or used as source of nutraceuticals.

Highlights

Hypertension is related to the incidence of coronary heart disease and its treatment is effective in reducing the risk of the disease [1]
The present study investigated angiotensin-I-converting enzyme (ACE) inhibitory and antioxidant activities of barbel muscle protein hydrolysate prepared with Alcalase
Several reports have been published on the ACE inhibitory activity of peptides from food proteins, like casein [4], mushroom [5], whey protein [6], soybean [7], and fish proteins [8]

Summary

Introduction

Hypertension is related to the incidence of coronary heart disease and its treatment is effective in reducing the risk of the disease [1]. The new fisheries management constraint is how to adopt new development strategies coproducts They are mostly converted into the meal and oil but it is a low valueadded processing. Enzymatic hydrolysis applied to marine products in order to produce bioactive peptides is one of the possible ways to effectively use these resources This process allows recovery of proteins that retain their content of essential amino acids and peptides which may have biological functions. Within this context, the present work was carried out aiming to study angiotensin-I-converting enzyme inhibitory and antioxidant activities of barbel muscle protein hydrolysate obtained by enzymatic treatments

Materials and Methods

Antioxidant Activity

Results and Discussion

Antioxidant Activity of BMPH

Conclusion