Abstract
The purpose of this study was to determine the angiotensin converting enzyme (ACE) inhibitory and antioxidant activities of myofibrillar protein hydrolysates (HMPHs) of different molecular weights (<3 and <10 kDa) derived from Korean native cattle (Hanwoo breed) using a commercially available and inexpensive enzyme (Alkaline-AK). HMPH of both tested molecular weights had ACE inhibitory activity. Among the antioxidant activities, iron chelation and nitrite scavenging activities were higher in low-molecular-weight peptide of HMPH (<3 kDa), whereas 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was higher in high-molecular-weight peptide of HMPH (<10 kDa). HMPH did not induce cytotoxicity in RAW 264.7 cells at concentrations of 5โ20 mg/mL. These results indicate that HMPH can be cheaply produced using Alkaline-AK and applied as a potential ACE inhibitor and antioxidant.
Highlights
Numerous studies have focused on bioactive peptides isolated from food sources and their diverse properties such as antioxidant, hypotensive, antimicrobial, immunomodulatory, antithrombotic, and opioid receptor-activating activities [1,2,3]
These results indicate that the hydrolysis efficiency of Alkaline-AK seems to be the same as that of pepsin (Figure 1)
This study determined that peptides of HMPH have an Angiotensin converting enzyme (ACE) inhibitory effect and an antioxidant activity
Summary
Numerous studies have focused on bioactive peptides isolated from food sources and their diverse properties such as antioxidant, hypotensive, antimicrobial, immunomodulatory, antithrombotic, and opioid receptor-activating activities [1,2,3]. Bioactive peptides have been produced from food sources using various proteases such as pepsin, trypsin alcalase, neutrase, and thermolysin [1, 4, 5]. Angiotensin converting enzyme (ACE) inhibitory and antioxidative peptides can be obtained from meat protein, which contains a high abundance of certain essential amino acids that are sparse in plant protein [6, 7]. Several studies [6, 9, 10] have reported that antioxidant peptides derived from meat protein are safer than synthetic antioxidant agents for in vivo application. Few studies on peptides derived from beef protein have been reported
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
