Angiopoietin-like protein 1 inhibits epithelial to mesenchymal transition in colorectal cancer cells via suppress Slug expression.

Abstract

The role of ANGPTL1 in cancer development is still little known, especially in colorectal cancer (CRC). We investigated the clinical significance of ANGPTL1 expression in CRC tissues and its potential role in the progression of epithelial to mesenchymal transition (EMT) in CRC cells, which has not been reported to our knowledge. ANGPTL1 expression in CRC tissues was much lower that than in paired adjacent normal tissues by IHC, WB and qRT-PCR assays. ANGPTL1 positive expression was negatively associated with tumor size (P = 0.034), T stage (P = 0.015), lymph nodes metastasis (P = 0.045) and TNM stage (P = 0.009) and poor prognosis of CRC patients (P = 0.003). In vitro, ANGPTL1 showed decreasing expression in CRC cell lines from primary tumor to ascites metastasis. Meanwhile, ANGPTL1 silencing enhanced EMT in HCT116 cells followed with the increase of Slug, Fibronectin and Vimentin, the decrease of E-cad, and the enhancement of EMT-like cell morphology and cell invasion and migration. Low ANGPTL1 expression is closely associated with multiple clinical significance and prognosis of CRC patients. ANGPTL1 inhibits EMT of CRC cells via inhibiting E-cad suppressor Slug expression.