Abstract
Angiogenesis is a crucial step in tissue regeneration and repair. Biomaterials that allow or promote angiogenesis are thus beneficial. In this study, angiogenic properties of salt-leached silk fibroin (SF) scaffolds seeded with human adipose stem cells (hADSCs) were studied using chick chorioallantoic membrane (CAM) as a model. The hADSC-seeded SF scaffolds (SF-hADSC) with the porosity of 77.34 ± 6.96% and the pore diameter of 513.95 ± 4.99 µm were implanted on the CAM of chick embryos that were on an embryonic day 8 (E8) of development. The SF-hADSC scaffolds induced a spoke-wheel pattern of capillary network indicative of angiogenesis, which was evident since E11. Moreover, the ingrowth of blood vessels into the scaffolds was seen in histological sections. The unseeded scaffolds induced the same extent of angiogenesis later on E14. By contrast, the control group could not induce the same extent of angiogenesis. In vitro cytotoxicity tests and in vivo angioirritative study reaffirmed the biocompatibility of the scaffolds. This work highlighted that the biocompatible SF-hADSC scaffolds accelerate angiogenesis, and hence they can be a promising biomaterial for the regeneration of tissues that require angiogenesis.
Highlights
Angiogenesis is needed for the regeneration of various tissues [1]
All animal procedures were approved by Chulalongkorn University Animal Care and Use Protocol (CU-ACUP), Faculty of Pharmaceutical Sciences, Chulalongkorn University (Protocol no. 1633008)
We found that the silk fibroin (SF) scaffolds were highly porous, and the pores were interconnected
Summary
Angiogenesis is needed for the regeneration of various tissues [1]. Two types of angiogenesis are known: sprouting and royalsocietypublishing.org/journal/rsos R. Sprouting angiogenesis starts from vasodilation, degradation of the 2 basement membrane, proliferation and migration of endothelial cells (ECs) from existing blood vessels [2]. The interplay between those processes and inflammation or hypoxia in wounded tissue generates pro-angiogenic factors that promote active proliferation and chemotactic migration of ECs toward the wounded area [1,2,3]. This results in the formation of new blood vessels. This type of angiogenesis relies mainly on EC reorganization rather than proliferation [4]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
