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Abstract
Leukocyte- and Platelet-Rich Fibrin (L-PRF) is an autologous platelet concentrate, consisting of a fibrin matrix enriched with platelets, leukocytes and a plethora of cytokines and growth factors. Since L-PRF is produced bedside from whole blood without the use of an anti-coagulant, it is becoming a popular adjuvant in regenerative medicine. While other types of platelet concentrates have been described to stimulate blood vessel formation, little is known about the angiogenic capacities of L-PRF. Therefore, this study aimed to fully characterize the angiogenic potential of L-PRF. With an antibody array, the growth factors released by L-PRF were determined and high levels of CXC chemokine receptor 2 (CXCR-2) ligands and epidermal growth factor (EGF) were found. L-PRF induced in vitro key steps of the angiogenic process: endothelial proliferation, migration and tube formation. In addition, we could clearly demonstrate that L-PRF is able to induce blood vessel formation in vivo, the chorioallantoic membrane assay. In conclusion, we could demonstrate the angiogenic capacity of L-PRF both in vitro and in vivo, underlying the clinical potential of this easy-to-use platelet concentrate.
Highlights
The first part of this study focused on investigating the growth factor release from Leukocyte- and Platelet-Rich Fibrin (L-PRF)
An antibody array was performed in order to obtain a more general screening of the growth factors that are released from L-PRF (Fig. 1A,B)
Analysis indicated high protein levels of epidermal growth factor (EGF) present in L-PRF conditioned medium (CM) compared to L-PRF EX
Summary
This study aimed to fully characterize the angiogenic potential of L-PRF. The aim of the present study is to evaluate growth factor release of L-PRF and to determine its effect on endothelial cell proliferation, migration and tube formation in vitro.
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