Abstract
Adipose‐derived microvessel fragments (MFs) form a mature, perfused microvessel network when implanted in SCID mice. However, adipose tissue is rich in resident mesenchymal cells that could uniquely drive spontaneous neovascularization from adipose derived MFs. Therefore, we evaluated if a more specialized vascular bed, lacking these precursor cells, would recapitulate MF angiogenesis and neovascularization. Microvessel fragments isolated from rodent brain cortex (BMFs) suspended in collagen gels were either cultured or implanted into host mice. Characterization of BMFs showed the presence of associated mural cells but not astrocytes. In vitro, these fragments form a neovessel network by undergoing sprouting angiogenesis similar to adipose‐derived MFs. When implanted for 28 days, brain‐derived MFs formed a microvasculature similar to adipose‐derived MFs which was functionally connected to the host circulation as demonstrated by FITC‐lectin perfusion. Importantly, implanted BMFs retained staining for Glut1 receptor, considered a marker of brain endothelium, and exhibited reduced macromolecular vessel permeability. This demonstrates that there is an intrinsic ability for isolated microvessel elements to neovascularize which is independent of the tissue origin and degree of vessel specialization. Supported by NIH R01 EB007556.
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