Abstract
β-galactosidase encoded by lacZ remains a popular reporter enzyme. Here, we present a convenient tool box to facilitate rapid construction of reporter lacZ fusions. The first tool enables the simple generation of chromosomally encoded reporters within the Escherichia coli lac operon using Red®/ET® recombination. The second tool allows the introduction of lacZ reporter fusions into the chromosome by a single-crossover method. The third tool comprises four pBBR1-based broad-host-range vectors to derive transcriptional and translational lacZ fusions.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.