Abstract
β-galactosidase encoded by lacZ remains a popular reporter enzyme. Here, we present a convenient tool box to facilitate rapid construction of reporter lacZ fusions. The first tool enables the simple generation of chromosomally encoded reporters within the Escherichia coli lac operon using Red®/ET® recombination. The second tool allows the introduction of lacZ reporter fusions into the chromosome by a single-crossover method. The third tool comprises four pBBR1-based broad-host-range vectors to derive transcriptional and translational lacZ fusions.
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