Angelica archangelica essential oil loaded chitosan nanoemulsion as edible coating for preservation of table grape fruit against Botrytis cinerea contamination and storage quality deterioration

Abstract

The present study deals with the encapsulation of Angelica archangelica essential oil into chitosan nanoemulsion (AAEO-NE) and evaluation of its effectiveness as novel coating agent with innovative controlled release delivery system for protection of table grape (Vitis vinifera L.) fruit against Botrytis cinerea contamination, and quality deterioration over 30 d of storage (25 ± 2 °C, Relative humidity 85%). The AAEO-NE was characterized through dynamic light scattering (DLS), scanning electron microscopy (SEM), and atomic force microscopy (AFM). The mean particle size was tended to increase from 53.69 to 98.63 nm, while ζ-potential was decreased from + 32.3 to + 23.5 mV. Fourier transform infrared spectroscopy (FTIR), and X-ray diffractometry (XRD) analyses suggested intermolecular interaction followed by effective encompassment of AAEO into chitosan nanomatrix. In vitro release study confirmed the biphasic and controlled delivery of AAEO, essentially required for its long term effectiveness in view of maintaining postharvest quality of the treated fruit. The antifungal efficacy of unencapsulated AAEO against B. cinerea was found as 5.5 g L−1, and noticed to be enhanced (2.5 g L−1) after encapsulation into chitosan nanoemulsion. The inhibition of ergosterol biosynthesis followed by subsequent enhancement of ions leakage after treatment of B. cinerea cells with AAEO-NE suggested plasma membrane as major site of antifungal activity. During in vivo investigation, AAEO-NE coating inhibited the contamination of B. cinerea and maintained the quality attributes like weight, titrable acidity, total soluble solids, phenolic content, and pH of table grape fruit. Additionally, the preservation of enzymatic antioxidants, reduced respiration rate, and better sensory qualities of coated table grape fruit recommend the functional potentiality of chitosan as biocompatible wall matrix to encapsulate AAEO and application as novel nano-green smart coating based fungitoxicant and shelf-life enhancer in fruit and agricultural industries.