Abstract
In this study, we aimed to compare DNA fragmentation ratios, centrosomal protein concentrations (centrin and tubulin) and numerical chromosomal abnormalities between four groups including asthenozoospermia, teratozoospermia, oligoasthenozoospermia and normozoospermia patients, and to emphasize the necessity of investigating genetic and protein content of the spermatozoa also in patients with a semen analysis within normal reference range. Evaluation of semen analysis of the patients was performed based on WHO 2010 criteria. As a result of the statistical analysis, it was observed that the sperm DNA fragmentation rate in the normozoospermia group (6.50 ± 3.46%) was significantly lower than those in the teratozoospermia (19.69 ± 9.86%) and oligoasthenoteratozoospermia group (32.47± 14.13%) (p<0.001). For centrin and tubulin proteins, highest concentrations were observed in normozospermia group (p<0.05). When incidence of numerical abnormalities were compared between four groups, it was found that there were statistically significant differences between groups in terms of nullisomies X, Y and 13, and 21 (p<0.05). In conclusion, we suggest that abnormalities in semen parameters seem to be associated with chromosomal abnormalities; and sperm aneuploidy rate is also at a considerable level even in normozoospermic patients. Keywords: DNA fragmentation, teratozoospermia, centrin, tubulin DOI: 10.7176/JMPB/68-04 Publication date: September 30 th 2020
Highlights
IntroductionIn studies performed to date, sperm chromatin defects and DNA fragments in infertile men have been shown to be higher than in fertile patients (Zini, 2001; Agarwal,2003; O Brien,2005; Schulte,2010; Omran,2013)
Standard semen analysis is routinely used in the evaluation of male infertility, the evaluated parameters may be insufficient in the exact separation of fertile and infertile men (Natali, 2011)
It has been reported that most of www.iiste.org the chromosome anomalies that occur after ICSI, which is one of the assisted reproductive techniques performed using the sperm of infertile men, are from paternal origin [Spano,2000,Mehdi,2006)
Summary
In studies performed to date, sperm chromatin defects and DNA fragments in infertile men have been shown to be higher than in fertile patients (Zini, 2001; Agarwal,2003; O Brien,2005; Schulte,2010; Omran,2013). Another point is the positive or negative effects of the nuclear quality of spermatozoon, especially in the success of assisted reproductive techniques applied in infertile couples. High aneuploidy levels have been reported in fragmented DNA-containing spermatozoa of infertile cases with abnormal semen parameters (Brahem,2012), there are studies showing that there is no relationship (Tandara,2014). We aimed to compare DNA fragmentation ratios, centrosomal protein concentrations (centrin and tubulin) and numerical chromosomal abnormalities between four groups including asthenozoospermia, teratozoospermia, oligoasthenoteratospermia and normozoospermia patients and to emphasize the necessity of investigating genetic and protein content of the spermatozoa in patients with a semen analysis within normal reference range
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