Aneuploidy and mosaicism concordance between cell-free embryonic DNA in spent embryo culture medium combined with blastocoel fluid and trophectoderm biopsy DNA

Abstract

Background and Aim: Some mosaic blastocysts are capable of forming viable normal pregnancies. However, the association between mosaicism in trophectoderm (TE) and the inner cell mass (ICM) is far from being established. Unlike trophectoderm biopsy, non-invasive or minimally invasive preimplantation genetic testing for aneuploidy (miPGT-A) may better represent the entire blastocyst since cell-free embryonic DNA is most likely released into blastocoel fluid (BF) and spent embryo culture medium (SEM) from both TE and ICM.