Abstract

As an anesthetic for long-term anesthesia, two concentrations of CO2 were used: a high concentration (Pco2 ?? 200, 225, or 250mmHg) for inducing anesthesia quickly and a low concentration (Pco2 ?? 100mmHg or 125mmHg) for maintaining anesthesia. Adult carp were kept in water containing the high or low concentration of CO2 for a total of 10h; the duration of each treatment was 15 min or 30 min at the high concentration and 1h or 9.5h at the low concentration. The time needed for the induction of anesthesia was shortened by the use of the high partial pressure of CO2 (Pco2 ?? 250mmHg). The carp could be anesthetized or sedated for 10h, using Pco2=250mmHg as the high concentration and Pco2=100mmHg as the low concentration of CO2.

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