Andrographis paniculata and Its Bioactive Diterpenoids Against Inflammation and Oxidative Stress in Keratinocytes.

Eugenie Mussard,Brigitte Legrain,Sundy Jousselin,Eric Lespessailles,Eric Esteve,Hechmi Toumi,Annabelle Cesaro,Sabine Berteina-Raboin

doi:10.3390/antiox9060530

Abstract

Andrographis paniculata was widely used in traditional herbal medicine to treat various diseases. This study explored the potential anti-aging activity of Andrographis paniculata in cutaneous cells. Human, adult, low calcium, high temperature (HaCaT) cells were treated with methanolic extract (ME), andrographolide (ANDRO), neoandrographolide (NEO), 14-deoxyandrographolide (14DAP) and 14-deoxy-11,12-didehydroandrographolide (14DAP11-12). Oxidative stress and inflammation were induced by hydrogen peroxide and lipopolysaccharide/TNF-α, respectively. Reactive oxygen species (ROS) production was measured by fluorescence using a 2′,7′-dichlorofluorescein diacetate (DCFH-DA) probe and cytokines were quantified by ELISA for interleukin-8 (IL-8) or reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for tumor necrosis factor-α (TNF-α). Hyaluronic acid (HA) secretion was determined by an ELISA. Our results show a decrease in ROS production and TNF-α expression by ME (5 µg/mL) in HaCaT under pro-oxidant and pro-inflammatory conditions, respectively. ME protected HaCaT against oxidative stress and inflammation. Our findings confirm that ME can be used for the development of bioactive compounds against epidermal damage.

Highlights

The skin is the largest human organ, the interface between the body and the environment
ANDRO was identified in Methanolic extract (ME) at 11.9 min by comparing the retention times obtained using a standard
We detected a proportion of ANDRO in ME of 0.87% (Figure 1)

Summary

Introduction

The skin is the largest human organ, the interface between the body and the environment. The skin suffers natural wear and tear, just like the rest of our body Skin aging has both intrinsic and extrinsic origins [1,2]. The former correspond to the fiber meshwork of collagen and elastin (intrinsic aging) and the latter to the environmental factors that age our skin such as ultraviolet light. In both processes, oxidative stress plays a role in inflammation induction and the destruction of skin proteins [3,4]. This results in a decrease in the renewal of epidermal cells and a decrease

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Discussion

Conclusion