Abstract
Androgens in human amniotic fluid were analyzed to determine their r elationship to the sex of the newborn. 43 samples were obtained by amni ocentesis close to term to analyze for 17beta-hydroxyandrogens. The antiserum for the radioimmunoassay was prepared against testosterone-3-b ovine serum albumin and it cross-reacted markedly only with 17beta-hydroxy-5alpha-androstan-3-one. Therefore the method was considered as measuring total immunoreactive 17beta-hydroxyandrogens tho ugh testosterone is probably the major steroid present. Comparison of this method with a protein-binding method for testosterone gave a correlation coefficient of .973. Sex of the newborn was ascertained from medical records after the assays were carried out. A significant difference between males and females was seen (p less than .005) but sexes could not be distinguished in a majority of individual cases. The difference between the 2 sexes was attributed to the percentage distribution of the values. It is concluded that 17beta-hydroxyandrogen s in amniotic fluid obtained close to term cannot be used to determine the sex of the fetus.
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