Abstract
Androgenesis in vitro is a basic method of obtaining haploid plants and DH (doubled haploid) lines of major crops such as potato, rapeseed, tomato, pepper, wheat, maize, and barley, and also many different minor crops and species with lower agricultural impact. Diploid plants appearing among androgenic regenerants are the effect of spontaneous doubling of the chromosome number in haploid cells during an embryo’s early developmental stages and are valuable fully homozygous breeding material. The subject of the presented research is spontaneous diploidization occurring in the development of androgenic, haploid pepper regenerants. In the presented experiment, the formation of diploid seeds was observed in the progeny of an androgenic, haploid plant derived in an anther culture of a hybrid (Capsicum annuum L. ATZ × Capsicum annuum L. ‘Corno di toro’)F2. Agromorphological and molecular analyses concerned eight diploid plants being progeny of the anther-derived haploid regenerant. Five of the plants constituted a phenotypically balanced group with valuable agromorphological features. Their genetic homogeneity was confirmed using 10 RAPD markers and 16 ISSR markers. Based on the results, it was concluded that anther-derived haploid plants of Capsicum can be the source of diploid, apomictic seeds, and the obtained offspring may constitute genetically stable, valuable breeding material.
Highlights
The biggest challenge of modern agriculture is the intensification of agricultural production while limiting its negative impact on the environment
The cytometric analysis of the regenerants showed that one plant was haploid, and the other was diploid (Figure 2)
16 ISSR primers were used, and 97 monomorphic products were obtained, which showed genetic homogeneity of the offspring of anther-derived haploid regenerant. This is valuable information, because it extends the use of androgenic pepper regenerants in breeding programs and proves that anther-derived haploid plants of Capsicum can be the source of diploid and apomictic seeds, and the obtained offspring may constitute genetically stable breeding material
Summary
The biggest challenge of modern agriculture is the intensification of agricultural production while limiting its negative impact on the environment. Pepper fruits are a rich source of flavonoids, provitamin A, vitamin E, vitamin C, potassium, calcium, magnesium, and iron. They contain especially valuable lycopene and capsaicin, which help lower blood pressure and protect against cardiovascular diseases. A wide range of existing varieties of peppers with different tastes, colors, and fruit shapes is an invaluable source of genetic variation. The androgenic origin of diploid regenerants resulting from the spontaneous doubling of the haploid number of chromosomes is confirmed on the basis of morphological homogeneity in R2 and the subsequent generations [10]. The research presents the morphological and molecular characteristics of the second generation originating from seeds of an anther-derived haploid plant. The aim was to check the level of functional traits of the studied population and to verify the genetic homogeneity of the analyzed materials in the context of their use in the breeding of Capsicum annuum L
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
