Androgen receptors in the brain of neonatal normal male and androgen insensitive rats

Abstract

Androgen binding was investigated in the cytosol of brains from neonatal normal and tfm rats using sucrose gradient and charcoal assay. The neonatal normal rat brain contains androgen receptors which sediment at 8S in a sucrose gradient at low ionic strength and at 4S in the presence of 0.5M KCl. These receptors have a high affinity (Kd = 1.2 × 10 −9M) for dihydrotestosterone (DHT), with a binding capacity of 7.3 × 10 −15 moles/mg cytosol protein. By contrast, there is very little, if any, high affinity androgen receptors in the neonatal tfm rat brain. Apparently, metabolism of testosterone does not affect androgen binding in the neonatal rat brain. The presence of these receptors and the occurrence of aromatization in the brain of neonatal rats are discussed in relation to their possible roles in sexual differentiation of the brain.