Androgen receptor complex binding in murine skeletal muscle nuclei

Abstract

Examination of binding of androgen-receptor complexes from murine skeletal muscle cytosol was performed by modified nuclear retention assay and modified nuclear acceptor assay. The experiments showed the binding of androgen-receptor complexes to the nuclear acceptor sites to be a cooperative process. Hill analysis of the data obtained resulted in a Hill coefficient of 3,6. The apparent dissociation constant for binding of cytosolic [ 3H]-testosterone-receptor complexes to nuclei was found to be in the range of K D = 6 − 8 × 10 −11 M. The nuclear matrix was able to bind androgen-receptor complexes in a saturable way, too.