Abstract
We have studied androgen metabolism in L6 rat myoblasts. 4-androstene-3,17-dione (Adione), testosterone, 5α-dihydrotestosterone (DHT), and 5α-androstane-3α,17β-diol (3α-diol) were used for substrates and the amounts of metabolites formed from the respective substrates in the medium were determined. Conversion of Adione to testosterone was dominant over the reverse conversion. DHT formation from testosterone was low and did not change with the duration of incubation, whereas 3α-diol formation increased in a time-dependent manner. Major metabolite of testosterone was not DHT but 3α-diol. A large amount of 3α-diol was formed from DHT, however DHT formation from 3α-diol was very low. These data indicate that L6 cells have high 5α-reductase activity and suggest that DHT formed from testosterone is rapidly metabolized to 3α-diol in these cells.
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