ANDP-2, A Novel Acrinol Degradation Product by Light

Abstract

To clarify the degradation pathway of acrinol by light, isolation and identifi cation of acrinol degradation products (ANDP) were attempted. ANDP2, on eo ft he ANDPs, was isolated by extraction with methanol from cloths dampened with acrinol solution, and purified by column chromatography on Diaion HP-10, Sephadex LH-20 and G25. Th es tructural elucidation of ANDP-2 was examined by infrared, 1 H-NMR, 13 C-NMR and Fast atom bombardment mass (FAB-MS) spectra studies. From the spectroscopic data, the structure of ANDP-2 was determined to be 4-amino-6-ethoxyl-2,3dicarboxyquinoline, that was found to be a novel degradation product of acrinol by light. The solution of ANDP-2 has fluorescence with a blue color, though that of acrinol has fluorescence with green. ANDP-2 did not show growth inhibition at a concentration of 100µg/ml against Gram-positive, -negative bacteria, yeast and fungi.