Abstract
The fruits from the Chilean Podocarpaceae Prumnopitys andina have been consumed since pre-Hispanic times. Little is known about the composition and biological properties of this fruit. The aim of this work was to identify the secondary metabolites of the edible part of P. andina fruits and to assess their antioxidant activity by means of chemical and cell-based assays. Methanol extracts from P. andina fruits were fractionated on a XAD7 resin and the main compounds were isolated by chromatographic means. Antioxidant activity was determined by means of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), ferric reducing power (FRAP), trolox equivalent antioxidant capacity (TEAC) and oxygen radical absorbance capacity (ORAC) assays. The cytoprotective activity of the extract against oxidative and dicarbonyl stress was evaluated in human gastric epithelial cells (AGS). The total intracellular antioxidant activity (TAA) of the extract was determined in AGS cells. The inhibition of meat lipoperoxidation was evaluated under simulated gastric digestion conditions. Rutin, caffeic acid β-glucoside and 20-hydroxyecdysone were identified as major components of the fruit extract. Additional compounds were identified by high-performance liquid chromatography diode-array detector mass spectrometry (HPLC-DAD-MSn) and/or co-injection with standards. Extracts showed dose-dependent cytoprotective effects against oxidative and dicarbonyl-induced damage in AGS cells. The TAA increased with the pre-incubation of AGS cells with the extract. This is the first report on the composition and biological activity of this Andean fruit.
Highlights
The Podocarpaceae are gymnosperms occurring mainly in the Southern Hemisphere and comprise20 genera and about 190 species [1]
Extract chromatogram of the P. andina aril extract retained in the resin Amberlite XAD7 showed a fingerprint fromaP.main elatuscompound did not protect oxidativeultraviolet stress [9]. (UV)
A total of 24 compounds were detected in the fruit extract from P. andina
Summary
The Podocarpaceae are gymnosperms occurring mainly in the Southern Hemisphere and comprise. 20 genera and about 190 species [1]. Most studies on this plant family have been carried out on the genus. Podocarpus, including species from Australia, Asia and South Africa [2], with a focus on constituents from the bark, stem and leaves. The arils from the Australian and New Zealand species Podocarpus dacrydioides, P. neriifolius, P. nivalis, P. salignus and P. totara are eaten raw or cooked. In Australia, the Molecules 2019, 24, 4028; doi:10.3390/molecules24224028 www.mdpi.com/journal/molecules. The fleshy stem of Podocarpus elatus is eaten by the Australian Aborigine and the fruits are consumed raw or cooked [2]. The sweet fruits from the Chilean native Podocarpaceae Prumnopitys andina Poepp.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
