Abstract
SP-28 In order to investigate the origin of HTLV-I prevailing among Andes Mongoloids, we isolated ancient HTLV-I provirus DNA from mummy bone marrow which were preserved in San Pedro de Atacama of North Chile. A total of 104 cases of mummy bone marrow DNA were rendered for PCR amplification of HTLV-I pX, β-globin and HLA-DQB1 genes. We successfully amplified an ancient HTLV-I pX DNA sequence from one of 104 mummies by prevention the cross-contamination from modern DNAs. The nucleotide sequence of the ancient HTLV-I provirus DNA showed very little variation in the 159 bp of HTLV-I pX gene as compared with contemporary HTLV-I provirus DNA sequence form Japanese and Chilean native HTLV-I carriers. We were also able to amplify 112 bp of β-globin gene and 267 bp of HLA DNA sequence relevant to HLA-DQB1*0502 from mummy DNAs. This is the first demonstration that ancient Andes mummy was infected with HTLV-I more than 1,500 years ago among aboriginal Andean Mongoloids migrated from the Asian Continent carrying with HTLV-I.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
