Ancient cattle DNA provides novel insight into the subsistence mode transition from the late Neolithic to Bronze Age in the Nenjiang River Basin

Abstract

The Honghe site (Heilongjiang Province, Northeast China) is located in the middle reaches of the Nenjiang River Basin, which situates rich cultural relics and faunal remains dating between the Neolithic and Bronze ages. The site hosts abundant bovine genus remains that are ideal for exploring the genetic history of the bovid species and the subsistence mode of the populations there. In this study, we recovered 14 mitochondrial genomes from 16 bovid samples selected for ancient DNA analysis. Phylogenetic analysis identified them as aurochs (Bos primigenius) and taurine cattle (Bos taurus), with one in eight Neolithic samples belonging to haplogroup T3, the most common type of domesticated cattle in ancient China. The remainder clustered with haplogroup C, found only in Chinese aurochs, while haplogroups C and T3 in six Bronze Age samples accounted for 50% each. Hence, it was speculated that domesticated cattle might have arisen in the middle reaches of the Nenjiang River Basin around 4000 years ago. However, the C-type aurochs remained among the essential resources for cattle production at the Angangxi people's disposal until the Bronze Age. Moreover, more T3-type taurine cattle were introduced during the Bronze Age. These domesticated cattle shared haplotypes with ancient cattle from the Northwest and Central Plains, showing close affinities, thus, reflecting frequent and extensive correlation between the middle reaches of the Nenjiang River and other regions.