Anatomy of olivocochlear neurons in the hamster studied with FluoroGold

Abstract

The golden hamster ( Mesocricetus auratus) is often used in auditory research, but little is known about the anatomical organization of its olivocochlear (OC) neurons, the source of the efferent innervation of the organ of Corti. Accordingly, we labeled the OC neurons projecting to one cochlea by means of retrograde axonal transport of FluoroGold ®. In four animals, all labeled OC neurons were counted and digital images of the labeling were captured and analyzed morphometrically. In one case, a 3D computer reconstruction of the bilateral distribution of OC neurons was made. The largest group of OC neurons was comprised by small, intrinsic lateral OC neurons within the ipsilateral lateral superior olivary nucleus (LSO), almost all of which (97%) were located ipsilaterally. The second largest group consisted of medial OC neurons in the ventral nucleus of the trapezoid body, 75% of which were located contralaterally. The smallest group consisted of shell neurons surrounding the LSO, 80% of which projected ipsilaterally. These three types of neurons are generally similar in morphology and distribution to those previously described in the rat and the chinchilla. However, there were several unique findings, including the fact that the hamster possesses the smallest total number of OC neurons (mean 341) of any rodent yet studied.