Anatomy and physiology of horizontal cells in layer 5b of the chicken optic tectum

Abstract

In the visual midbrain of birds, a variety of cell types has recently been characterized with both anatomical and physiological techniques to gain insight into the mechanisms of visual information processing. Here we present data from a horizontal cell type located in the retinorecipient layer 5b of the chick optic tectum. Intracellular labeling revealed that these neurons are multipolar, have no axonal structures and arborize completely within the layer 5b where they extend over considerable distances. Immunohistochemistry with an antibody against calbindin labeled a population of horizontal cells in layer 5b; however, double labeling showed that these neurons represent a subpopulation of approximately one third of the neurons in that layer. Whole-cell patch recordings with additional cell filling from horizontal cells revealed that the physiological responses to depolarization changes with maturation, from a comparatively slow oscillatory pattern reminiscent of hair cell physiology at embryonal stages to a damped series of small action potentials at posthatching. In response to electrical stimulation in the vicinity of the neurons, cells responded with either excitatory postsynaptic potentials or small action potentials. Horizontal cell types are found in the visual midbrain of both avian and mammalian species. On the basis of the data presented here and data from the literature, the functional role of these cells is discussed. As in layer 5b of the chick optic tectum specific synaptic glomeruli have been found, the horizontal cells might constitute local inhibitory circuits within the retino-tectal synapses and, in addition, contribute to mechanisms of directional selectivity in these projections.