Anatomo-radiological correlation using 18-FDG-PET in abdominal sepsis model in rats. A preliminary study.

Ítalo Medeiros Azevedo,Marília Daniela Ferreira Carvalho,Mônica Raquel De Souza Aquino,Robson Macedo,Rafael Pereira Nascimento,Aldo Cunha Medeiros

doi:10.1590/s0102-865020170030000001

Ítalo Medeiros Azevedo, Marília Daniela Ferreira Carvalho + Show 4 more

Open Access

https://doi.org/10.1590/s0102-865020170030000001

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Abstract

To examine a correlation of micro-PET images with photographic images of the digestive organs in abdominal sepsis model. Male Wistar rats weighing 265±18g were used. Abdominal sepsis was induced by ligature and cecal puncture. Micro-PET Images from abdominal cavity septic foci were obtained using 18-Fluoro-deoxyglucose, looking for a correlation with photographic images of abdominal cavity organs. Pearson's correlation test was used. The mean standard uptake values (SUV) and lesion areas were 2.58±0.63SUVbwg/ml and 546.87±300.95mm2, respectively. There was a strong positive correlation between the two variables (r=0.863, p=0.137), which resulted in a coefficient of determination r2?0.75, meaning that 75% of SUV variation is explained by the lesion areas of digestive organs. Micro-PET allows high throughput assessment of lesion count and volume in pre-clinical rat model of CPL abdominal sepsis.

Highlights

One of the major current public health problems remains sepsis, which persists with high hospital mortality
Establishing an order of effectiveness and complexity, conventional radiography, ultrasonography, computed tomography (CT) and nuclear magnetic resonance imaging (MRI) have been the most widely used imaging tests to attempt the diagnosis of abdominal sepsis
Four animals were submitted to abdominal sepsis by ligature and cecal puncture and, 24 hours later, were examined through Positron Emission Tomography and photographic images of the open abdominal cavity

Summary

Introduction

One of the major current public health problems remains sepsis, which persists with high hospital mortality. The diagnostic means necessary for a specific and accurate clinical evaluation to clarify whether or not the patients have sepsis has been insufficient in many cases[2]. Establishing an order of effectiveness and complexity, conventional radiography, ultrasonography, computed tomography (CT) and nuclear magnetic resonance imaging (MRI) have been the most widely used imaging tests to attempt the diagnosis of abdominal sepsis. Conventional radiography was very useful in the years 1970-90, and continues with high percentages of false positive and false negative results[2,3]. MRI, like ultrasonography, does not include the use of contrast media and does not expose patients to x-ray[10]

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