Anatomical Study of Nerium oleander L. Leaves Found in Places Contaminated with H2S

Abstract

The research study the effect of H2S on Nerium oleander leaves, the leaves were collected from the pollution places in the crowded streets with cars and factory areas from five different regions of Basrah city, The results showed that the average of highest concentration of hydrogen sulfide gas was 4. ppm in the Al-Amin Aldakhli area, while the lowest value of gas was recorded in Karmat Ali, it was 0.4 ppm of date palm plants and 0.8 ppm. Anatomy study of N. oleanderleaves showed that the different changes in plant internal tissues with H2S pollution which presented by difference in the thickness of epidermal cell walls of N. oleander leaves in the areas of Al-Dair, Manawi Pasha and Andal’s, while the thickness of the cells was less in the area of Karmat Ali. Highest average length of cells of the upper epidermis was recorded in the Al-Dier and Karmat Ali region was 40 μm and 37.5 μm respectively, while the lower epidermis was 35.82 μmin Al-Dier. Stomata dimension found the lowest average was recorded in the Al-Amin Aldakhli area was 24 μm while in the upper epidermis have highest value, it was in Karmat ali was 30 μm. The number of stomata different between the stations, it was found that the highest average of stomata was found in the lower epidermis in Al-Amin Aldakhli station was 215.98, while the lowest average in Karmat Ali was 98. The upper epidermis had the highest average number of stomata in the Minawi Pasha station was 183.96 on lower epidermis and the lowest average in Andal’s area was 66. Also, the highest average number of cells was in the Lower epidermis in Andal’s region was 3159.96, and the lowest average was 919.98 in the Al-Dair.