Abstract
Porang is one species member of the genus Amorphophallus . In Indonesia, Porang is cultivated extensively because it has a high economic value. There is three seeded material available for planting. One of seeded material is bulbil. Bulbil is a vegetative reproductive organ of Porang plants which is located in the branch of the leaf. Bulbil has 3 - 4 months dormancy period. The dormancy period will end with shoot growth. Morphologically, there can be found a rough structure in the form of bulges in bulbil. The bulge is spread throughout the bulbil surface and is a growing area of shoots. Shoots on bulbil will grow through the bulge and only grow on the adaxial part, contrary shoots do not grow from the abaxial part. This study aims to compare the anatomy of shoot apical meristem (SAM) in the adaxial and abaxial parts of bulbil at the end of the dormancy period, ie 100 days after harvest (DAH), 110 DAH, 120 DAH, and 130 DAH. Anatomical observations were carried out by making semi-permanent preparations. The results showed that the anatomy of the adaxial and abaxial parts of bulbil Porang had differences in the development of SAM, leaf primordia, primary thickening meristem (PTM) and procambium. Keywords : Anatomy, Bulbil Porang, Dormancy, Shoot Apical Meristem.
Highlights
INTRODUCTION* Porang (Amorphophallus muelleri Blume) is a member of Araceae [1] which in Javanese society is known asiles-iles [2]
Based on these empirical facts, this study aim to investigate the anatomy of bulbil both adaxial and abaxial parts after 3 months / 92 days after harvest (DAH) until the end of the dormancy period
The anatomical structure of bulbil at 100 DAH has been observed the presence of shoot apical meristem (SAM) in the adaxial part (Fig. 2A)
Summary
The bulbil used has approximately 92 DAH (3 months). The age was determined (calculated) since it was harvested from Porang. Shoot Apical Meristem on Bulbil Porang (Afifi et al) were reacheds 100 DAH, it begins to be soaked in the FAA solution followed periodically at 120 and 130 DAH in the same way. Observation of Bulbil Anatomy The whole bulbil were cut into two equal parts (adaxial and abaxial). Both parts were sliced vertically with a razor blade than each slice was sliced with a microtome (Figure 1). Sliced in the tubercle region intend to obtain the anatomical character of shoot apical meristem (SAM) in bulbil. The anatomy of bulbil was observed using semi-permanent slide which was preparations based on the modified Ruzin method [11]. The slides ready to observe with the light microscope and documented with a digital camera
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