Anatomical and physiological adjustments of pubescent oak (Quercus pubescens Willd.) from two adjacent sub-Mediterranean ecosites

Abstract

Patterns of drought severity in natural ecosystems depend on ecosite characteristics, particularly on local soil water availability and microclimate (evaporative demand). At the sites with less favourable conditions, the plasticity of fundamental drought adaptive traits can be exceeded under severe drought, even in well adapted species.In our study, anatomical and physiological adjustments to drought were studied in pubescent oak (Quercus pubescens Willd.) growing on adjacent sub-Mediterranean plots (ecosites) differing in bedrock and soil type (limestone with rendzic leptosol, low water retention - L; transition from limestone to flysch with eutric cambisol, high water retention - F).In both years of the study, 2015 and 2016, in the part of the season with sufficient water supply, sap flow density (SFD) was higher in oaks growing on L than those growing on F. With decreasing soil water content (SWC) in July, SFD was reduced on both plots, most in trees growing on limestone. In the period of severe drought, SFD in trees growing on L was decoupled from the changes of VPD, due to increased employment of water conservation strategies, such as decreases in stomatal conductance. This was further translated into stomatal limitation of photosynthesis and contributed to reduced radial stem growth.The seasonal dynamics of traits indicate that Q. pubescens trees use a predisposed anatomical strategy (average size of vessels in different years), adjusted to average ecosite conditions. At the same time, trees respond to local conditions and interanual variability of drought by shortening/prolonging the production and growth of vessels and by adjusting leaf functioning (stomatal conductance, sap flow) in accordance with hydraulic properties.