Anatomical and Histochemical Characterization of Leaves of Luffa cylindrica (L.) M. Roem

Laís Emanuelle Bernardo Vieira,Karina Perrelli Randau,Rafaela Damasceno Sá

doi:10.5530/pj.2019.11.81

Abstract

Background: Luffa cylindrica (L.) M. Roem. (Cucurbitaceae) is an herbaceous plant used for food as compose salads and do sweets and also used in traditional medicine as treat parasitic infections and intestinal diseases. Although this information, there are not many relates about anatomic characters for use in your quality control. Objective: The aim was investigating the anatomical characters of petiole and leaf blade and characterizing the metabolites in the leaf blade of L. cylindrica. Materials and Methods: Semipermanent histological slides were prepared for analysis of petiole and leaf blade in optical microscopy. Histochemical tests were also performed in the leaf blade. Results: The anatomical study revealed information about the type of trichomes, cuticle, vascular bundles and arrangement of the tissues that determine the botanical identity of this species. It was also identifying, for the first time, the presence of two types of trichomes in both of leaf blades faces. The histochemistry allowed determining which metabolites are in the leaf blade and also their location. Conclusion: The study described new characters for L. cylindrica and the results provide support to quality control of the species.

Highlights

Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Avenida Professor Arthur de Sá, Cidade Universitária, Recife, PE, BRAZIL
This research aimed to perform the anatomic and histochemistry characterization of leaves of L. cylindrica, in order to expend the pharmacobotanical information about species of Cucurbitaceae family
A voucher specimen was deposited in the Herbarium Dárdano de Andrade Lima of the Instituto Agronômico de Pernambuco (IPA), under collection number 92082

Summary

Introduction

Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Avenida Professor Arthur de Sá, Cidade Universitária, Recife, PE, BRAZIL. (Cucurbitaceae) is an herbaceous plant used for food as compose salads and do sweets and used in traditional medicine as treat parasitic infections and intestinal diseases This information, there are not many relates about anatomic characters for use in your quality control. Results: The anatomical study revealed information about the type of trichomes, cuticle, vascular bundles and arrangement of the tissues that determine the botanical identity of this species. It was identifying, for the first time, the presence of two types of trichomes in both of leaf blades faces.

Objectives

Methods

Results