Abstract

The article present results of the study for a anatomic structure of Campanula rotundifolia grass from Campanulaceae family. Despite its dispersion and application in folk medicine, there are no data about its anatomic structure, therefore to estimate the indices of authenticity and quality of raw materials it is necessary to develop microdiagnostical features in the first place, which could help introducing of this plant in a medical practice. The purpose of this work is to study anatomical structure of Campanula rotundifolia grass to determine its diagnostic features. Methods. The study for anatomic structure was carried out in accordance with the requirements of State Pharmacopoeia, edition XIII. Micromed laboratory microscope with digital adjutage was used to create microphotoes, Photoshop CC was used for their processing. Result. We have established that stalk epidermis is prosenchymal, slightly winding with straight of splayed end cells. After study for the epidermis cells we established that upper epidermis cells had straight walls and are slightly winding. The cells of lower epidermis have more winding walls with prolong wrinkled cuticule. Presence of simple one-cell, thin wall, rough papillose hair on leaf and stalk epidermis. Cells of epidermis in fauces of corolla are prosenchymal, with winding walls, straight or winding walls in a cup. Papillary excrescences can be found along the cup edges. Stomatal apparatus is anomocytic. Conclusion. As the result of the study we have carried out the research for Campanula rotundifolia grass anatomic structure, and determined microdiagnostic features for determination of raw materials authenticity, which included presence of simple, one-cell, thin-walled, rough papillose hair on both epidermises of a leaf, along the veins, leaf edge, and stalk epidermis, as well as the presence of epidermis cells with papillary excrescences along the edges of leaves and cups. Intercellular canals are situated along the veins of flower corolla petals.

Highlights

  • Campanula rotundifolia is a plurennial grassy plant from Campanulaceae family 15-60 cm high, which is possible to encounter in all areas of Central Russia as an ordinary plant

  • Despite its dispersion and application in folk medicine, there are no data about its anatomic structure, to estimate the indices of authenticity and quality of raw materials it is necessary to develop microdiagnostical features in the first place, which could help introducing of this plant in a medical practice

  • As the result of the study conducted we have examined the anatomic structure of Campanula rotundifolia grass and determined the microdiagnostic features to define the authenticity of raw materials

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Summary

Introduction

Campanula rotundifolia is a plurennial grassy plant from Campanulaceae family 15-60 cm high, which is possible to encounter in all areas of Central Russia as an ordinary plant. The plant grows on dry meadows, uncoverings of limestone мела, среди зарослей кустарников, а также на лесных опушках [1, 2]. Несмотря на широкий ареал распространения и применение колокольчика круглолистного в народной медицине, данные о его анатомическом строении отсутствуют, поэтому для объективной оценки показателей подлинности и качества сырья необходимо в первую очередь разработать микродиагностические признаки, которые могут позволить внедрить данное растение в медицинскую практику. Цель данной работы – изучить анатомическое строение травы колокольчика круглолистного с целью определения диагностических признаков. Объектом исследования являлась трава колокольчика круглолистного, заготовленная в период массового цветения на территории Курской области в 2015 году

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