Anastomosis groups and pathogenicity of binucleate Rhizoctonia isolates associated with stem canker of potato in China

Abstract

Potato stem canker caused by Rhizoctonia solani commonly occurs in potato-growing regions around the world, but little is known about whether binucleate Rhizoctonia (BNR) can incite this disease on potato plants in China. In the present study, a total of 69 BNR isolates were recovered from cankered subterraneous stems of potato collected from 17 provinces, autonomous regions, and municipalities of China. Based on the morphological characteristics and sequence analysis of the internal transcribed spacers of the ribosomal DNA, 48 isolates were classified as anastomosis group (AG)-A with a ratio of 69.56 %, 15 isolates (21.74 %) were AG-K, four isolates (5.80 %) were AG-F, one isolate (1.45 %) was AG-I and one isolate (1.45 %) was AG-U. Pathogenicity tests under glasshouse conditions revealed that all BNR isolates, except for the AG-I isolate, could induce symptoms of stem canker on potato plants with disease incidence ranging from 5.26 to 55.56 % and disease index ranging from 1.32 to 13.89, respectively. To our knowledge, this is the first detailed report about BNR AGs causing stem canker on potato plants in China.