Abstract
We conducted a 16S rRNA nested PCR for the genus Ehrlichia and Ehrlichia spp. with blood samples from 30 ill dogs in Chile. Phylogenetic analysis was performed by using groESL gene amplification. We identified Anaplasma platys as 1 of the etiologic agents of canine ehrlichiosis.
Highlights
We conducted a 16S rRNA nested PCR for the genus Ehrlichia and Ehrlichia spp. with blood samples from 30 ill dogs in Chile
Serologic and clinical evidence of canine ehrlichiosis and serologic evidence of human ehrlichiosis have been reported in Chile [3,4]
We identified A. platys DNA in the blood of 6 dogs with clinical signs indicative of ehrlichiosis
Summary
We conducted a 16S rRNA nested PCR for the genus Ehrlichia and Ehrlichia spp. with blood samples from 30 ill dogs in Chile. The brown dog tick, Rhipicephalus sanguineus, is the main tick that infests dogs in Chile [1] This tick species is a vector of Ehrlichia canis and has been implicated, but not confirmed, as a vector of Anaplasma platys [2]. The Ehrlichia genus PCR resulted in the expected DNA band in 6 of 30 dogs (dogs 7, 12, 17, 19, 23, and 25). These 6 samples were positive only for A. platys, showing the expected 151-bp product, and negative for other species tested (Figure 1, panel A). A. platys PCR was conducted on the remaining 24 Ehrlichia-negative samples; none were positive
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